ARG46759

anti-Puromycin antibody

anti-Puromycin antibody for Western blot and Other

Overview

Product Description Rabbit Polyclonal antibody recognizes Puromycin
Tested Reactivity Other
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Puromycin
Immunogen Chemical compounds corresponding to Puromycin.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 10-250 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen
Buffer PBS, 50% Glycerol, Proclin 300 or sodium azide
Preservative Proclin 300 or sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Puromycin
Background Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.

Images (2) Click the Picture to Zoom In

  • ARG46759 anti-Puromycin antibody WB image

    Western blot: 293F stained with ARG46759 anti-Puromycin antibody.

  • ARG46759 anti-Puromycin antibody WB image

    Western blot: RAW264.7 stained with ARG46759 anti-Puromycin antibody.