ARG46785

anti-RAB25 antibody

anti-RAB25 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes RAB25
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RAB25
Antigen Species Mouse
Immunogen Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse RAB25 produced in E. coli.
Conjugation Un-conjugated
Alternate Names RAB11C; CATX-8; CATX8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1,000
WB1:250 - 1:2,000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Epitope affinity purified
Buffer PBS, 20% Glycerol and 0.05% Sodium Azide
Preservative 0.05% Sodium Azide
Stabilizer 20% Glycerol
Concentration 2 mg/ml
Storage Instruction For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53868 Mouse RAB25

GeneID: 57111 Human RAB25

Swiss-port # P57735 Human Ras-related protein Rab-25

Swiss-port # Q9WTL2 Mouse Ras-related protein Rab-25

Gene Symbol RAB25
Gene Full Name RAB25, member RAS oncogene family
Background The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015]
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. RAB25 regulates epithelial cell differentiation, proliferation and survival. Promotes invasive migration of cells by localizing and maintaining integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway; together with MYO5B regulates transcytosis. [UniProt]
Cellular Localization Cell membrane; Cell projection; Cytoplasm; Cytoplasmic vesicle; Endosome; Membrane. [UniProt]
Calculated MW 23.5 kDa
PTM Lipoprotein; Methylation; Prenylation. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG46785 anti-RAB25 antibody WB image

    Western blot: Human breast stained with ARG46785 anti-RAB25 antibody.

  • ARG46785 anti-RAB25 antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG46785 anti-RAB25 antibody.

  • ARG46785 anti-RAB25 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG46785 anti-RAB25 antibody.

  • ARG46785 anti-RAB25 antibody IHC-P image

    Immunohistochemistry: Mouse intestine stained with ARG46785 anti-RAB25 antibody.