ARG45677
anti-RACK1 / GBN2L1 antibody
anti-RACK1 / GBN2L1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RACK1 / GBN2L1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RACK1 / GBN2L1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human RACK1 / GBN2L1. |
| Conjugation | Un-conjugated |
| Alternate Names | RACK1; Receptor For Activated C Kinase 1; GNB2L1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; HLC-7; H12.3; Gnb2-rs1; Cell proliferation-inducing gene 21 protein; Human lung cancer oncogene 7 protein; Receptor for activated C kinase; Guanine nucleotide-binding protein subunit beta-like protein 12.3; PIG21; Receptor of activated protein kinase C 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RACK1 |
| Gene Full Name | Receptor For Activated C Kinase 1 |
| Background | Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including regulation of protein metabolic process; regulation of signal transduction; and regulation of vesicle-mediated transport. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158). (Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. (Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs. (Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs. (Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses. [UniProt] |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Perikaryon. Cell projection, dendrite. Cell projection, phagocytic cup. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45677 anti-RACK1 / GBN2L1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45677 anti-RACK1 / GBN2L1 antibody at 5 μg/ml dilution.
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ARG45677 anti-RACK1 / GBN2L1 antibody WB image
Western blot: RAW264.7 stained with ARG45677 anti-RACK1 / GBN2L1 antibody at 0.5 μg/ml dilution.
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ARG45677 anti-RACK1 / GBN2L1 antibody FACS image
Flow Cytometry: A431 stained with ARG45677 anti-RACK1 / GBN2L1 antibody at 1 µg/10^6 cells dilution.
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ARG45677 anti-RACK1 / GBN2L1 antibody WB image
Western blot: Rat liver stained with ARG45677 anti-RACK1 / GBN2L1 antibody at 0.5 μg/ml dilution.
