ARG10620

anti-RBP4 antibody [RB48]

anti-RBP4 antibody [RB48] for Western blot,ELISA,Sandwich ELISA and Human

Overview

Product Description Mouse Monoclonal antibody [RB48] recognizes RBP4
Tested Reactivity Hu
Tested Application ELISA, WB, sELISA
Specificity This antibody recognizes free RBP4 and RBP4 in complex with transthyretin.
This antibody recognizes apo- as well as holo-RBP4.
Host Mouse
Clonality Monoclonal
Clone RB48
Isotype IgG1
Target Name RBP4
Antigen Species Human
Immunogen Human plasma RBP4.
Conjugation Un-conjugated
Full Name retinol binding protein 4, plasma
Alternate Names Plasma retinol-binding protein; MCOPCB10; RDCCAS; RBP; Retinol-binding protein 4; PRBP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
WBAssay-dependent
sELISAAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 5950 Human RBP4

Swiss-port # P02753 Human Retinol-binding protein 4

Gene Symbol RBP4
Gene Full Name retinol binding protein 4, plasma
Background This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Function Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. [UniProt]
Highlight Related products:
RBP4 antibodies; RBP4 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
A new role of fat – factory of circulating miRNAs

Images (3) Click the Picture to Zoom In

  • ARG10620 anti-RBP4 antibody [RB48] WB image

    Western blot: Immunodetection of RBP4 in WB after SDS electrophoresis in reducing conditions by ARG10620 anti-RBP4 antibody [RB48]. 1 µg of purified endogenous RBP4 was loaded.

  • ARG10620 anti-RBP4 antibody [RB48] sELISA image

    Sandwich ELISA: Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: ARG10620 anti-RBP4 antibody [RB48] (1 µg/well); Detection antibody: ARG10619 anti-RBP4 antibody [RB42] labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.

  • ARG10620 anti-RBP4 antibody [RB48] sELISA image

    Sandwich ELISA: Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: ARG10620 anti-RBP4 antibody [RB48] (1 µg/well); Detection antibody: ARG10619 anti-RBP4 antibody [RB42] labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.