anti-REDD1 phospho (Thr23 / Thr25) antibody

anti-REDD1 phospho (Thr23 / Thr25) antibody for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody


Product Description Rabbit Polyclonal antibody recognizes REDD1 phospho (Thr23 / Thr25)
Tested Reactivity Hu
Predict Reactivity Chimp
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name REDD1
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 conjugated to KLH
Conjugation Un-conjugated
Alternate Names DNA damage-inducible transcript 4 protein; REDD-1; REDD1; HIF-1 responsive protein RTP801; Dig2; Protein regulated in development and DNA damage response 1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~34k REDD1 phosphorylated at Thr23/25. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 54541 Human DDIT4

Swiss-port # Q9NX09 Human DNA damage-inducible transcript 4 protein

Gene Symbol DDIT4
Gene Full Name DNA-damage-inducible transcript 4
Background REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b (Katiyar et al, 2009).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 25 kDa
PTM Phosphorylated by GSK3B; this promotes proteasomal degradation.
Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.

Images (1) Click the Picture to Zoom In

  • ARG52412 anti-REDD1 phospho (Thr23 / Thr25) antibody WB image

    Western blot: Human jurkat lysate showing specific immunolabeling of the ~34k REDD1 protein phosphorylated at Thr 23/25 (control) stained with ARG52412 anti-REDD1 phospho (Thr23 / Thr25) antibody.
    Immunolabeling is blocked by the phospho-peptide used as antigen (peptide).