ARG20305

anti-RFP + dsRed antibody

anti-RFP + dsRed antibody for ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes RFP + dsRed
Tested Reactivity Other
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RFP + dsRed
Immunogen Recombinant RFP expressed from E. coli
Conjugation Un-conjugated
Alternate Names Red Fluorescent Protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 4 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3229046 Other DET_RS08290

Background Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Highlight Related Antibody Duos and Panels:
ARG30061 Tag Internal Control Antibody Duo (dsRed, GAPDH)
Related products:
RFP antibodies; RFP ELISA Kits; RFP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (1) Click the Picture to Zoom In

  • ARG20305 anti-RFP + dsRed antibody WB image

    Western blot: 1. Recombinant RFP (1000 ng), 2. Recombinant RFP (250 ng), 3. Recombinant RFP (50 ng), and 4. Recombinant RFP (10 ng) stained with ARG20305 anti-RFP + dsRed antibody.

Specific References

A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.

WB / Other (dsRed)

Murakami Manabu et al.
PLoS One.,  (2019)

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