ARG45673

anti-RHOT2 antibody

anti-RHOT2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RHOT2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RHOT2
Antigen Species Human
Immunogen Recombinant protein containing to human RHOT2.
Conjugation Un-conjugated
Alternate Names RHOT2; Ras Homolog Family Member T2; MIRO-2; C16orf39; MIRO2; ARHT2; Ras Homolog Gene Family, Member T2; Mitochondrial Rho (MIRO) GTPase 2; Mitochondrial Rho GTPase 2; Chromosome 16 Open Reading Frame 39; Ras Homolog Gene Family Member T2; EC 3.6.5.-; EC 3.6.5 47; HMiro-2; RASL

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
IP0.5-2 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 80 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 214952 Mouse RHOT2

GeneID: 287156 Rat RHOT2

GeneID: 89941 Human RHOT2

Gene Symbol RHOT2
Gene Full Name Ras Homolog Family Member T2
Background This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. [provided by RefSeq, Nov 2011]
Function Mitochondrial GTPase involved in mitochondrial trafficking. [UniProt]
Cellular Localization Membrane; Mitochondrion; Mitochondrion outer membrane. [UniProt]
Calculated MW 68 kDa

Images (8) Click the Picture to Zoom In

  • ARG45673 anti-RHOT2 antibody IHC-P image

    Immunohistochemistry: Human prostate cancer stained with ARG45673 anti-RHOT2 antibody at 2 μg/ml dilution.

  • ARG45673 anti-RHOT2 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45673 anti-RHOT2 antibody at 5 μg/ml dilution.

  • ARG45673 anti-RHOT2 antibody WB image

    Western blot: 293T stained with ARG45673 anti-RHOT2 antibody at 0.5 μg/ml dilution.

  • ARG45673 anti-RHOT2 antibody IP image

    Immunoprecipitation: MCF-7 lysate immunoprecipitated with 2 μg ARG45673 anti-RHOT2 antibody.

  • ARG45673 anti-RHOT2 antibody IP image

    Immunoprecipitation: MCF-7 lysate immunoprecipitated with 2 μg ARG45673 anti-RHOT2 antibody.

  • ARG45673 anti-RHOT2 antibody FACS image

    Flow Cytometry: MCF-7 stained with ARG45673 anti-RHOT2 antibody at 1 µg/10^6 cells dilution.

  • ARG45673 anti-RHOT2 antibody IHC-P image

    Immunohistochemistry: Rat stomach stained with ARG45673 anti-RHOT2 antibody at 2 μg/ml dilution.

  • ARG45673 anti-RHOT2 antibody IHC-P image

    Immunohistochemistry: Mouse stomach stained with ARG45673 anti-RHOT2 antibody at 2 μg/ml dilution.