anti-RIPK2 / RIP2 antibody
anti-RIPK2 / RIP2 antibody for ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes RIPK2 / RIP2|
|Tested Application||ICC/IF, WB|
|Specificity||This antibody recognizes full-length human, mouse and rat RIPK2 / RIP2 (60 kDa).|
|Target Name||RIPK2 / RIP2|
|Immunogen||Synthetic peptide corresponding to aa. 11-30 of Human RIPK2 / RIP2 (O43353).|
|Alternate Names||RIP-like-interacting CLARP kinase; Receptor-interacting protein 2; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; Receptor-interacting serine/threonine-protein kinase 2; RIP-2; GIG30; EC 220.127.116.11; CARDIAK; RIP2; RICK; CCK; CARD3; EC 18.104.22.168; Tyrosine-protein kinase RIPK2; CARD-containing IL-1 beta ICE-kinase|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Positive Control||A431 and K562|
|Buffer||PBS (pH 7.4) and 0.02% Sodium azide|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||receptor-interacting serine-threonine kinase 2|
|Background||A CARD-containing serine/threonine kinase that regulates apoptosis has been identified and designated RICK (RIP-like interacting CLARP kinase). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. RICK kinase domain has high sequence homology to that of RIP. Overexpression of RICK promotes the activation of caspase-8 and Fas-induced apoptosis. RICK represents a novel kinase that regulates Fas induced-apoptosis. The messenger RNA of RICK is expressed in a variety of human tissues.|
|Function||Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. [UniProt]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody|
|Calculated MW||61 kDa|
|PTM||Autophosphorylated. Autophosphorylation at Tyr-474 is necessary for effective NOD2 signaling. Phosphorylated. Phosphorylation at Tyr-381 by Src kinase CSK occurs in a ARHGEF2-dependent manner and is required for NOD2-dependent innate immune activation.
Ubiquitinated on Lys-209; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Also undergoes 'Met-1'-linked polyubiquitination; the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation. Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent proinflammatory signaling activation of NF-kappa-B.
Images (2) Click the Picture to Zoom In
ARG54416 anti-RIPK2 / RIP2 antibody ICC/IF image
Immunofluorescence: A431 cells stained with ARG54416 anti-RIPK2 / RIP2 antibody at 10 μg/ml dilution.
ARG54416 anti-RIPK2 / RIP2 antibody WB image
Western blot: A431 (A) and K562 (K) cell lysates stained with ARG54416 anti-RIPK2 / RIP2 antibody at 1 μg/ml dilution.