ARG45642
anti-RNF25 antibody
anti-RNF25 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RNF25 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RNF25 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human RNF25. |
| Conjugation | Un-conjugated |
| Alternate Names | RNF25; Ring Finger Protein 25; AO7; E3 Ubiquitin-Protein Ligase RNF25; FLJ13906; RING-Type E3 Ubiquitin Transferase RNF25; Ring Finger Protein AO7; RING Finger Protein AO7; RING Finger Protein 25; EC 2.3.2.27 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 62 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RNF25 |
| Gene Full Name | Ring Finger Protein 25 |
| Background | The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq, Jul 2008] |
| Function | E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45642 anti-RNF25 antibody IHC-P image
Immunohistochemistry: Human lung adenocarcinoma stained with ARG45642 anti-RNF25 antibody at 2 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Hela and U251 stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
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ARG45642 anti-RNF25 antibody FACS image
Flow Cytometry: 293T stained with ARG45642 anti-RNF25 antibody at 1 µg/10^6 cells dilution.
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ARG45642 anti-RNF25 antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG45642 anti-RNF25 antibody at 2 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Rat testis stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Mouse testis stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
