anti-ROS1 antibody [SQab22273]

anti-ROS1 antibody [SQab22273] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab22273] recognizes ROS1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22273
Isotype IgG
Target Name ROS1
Antigen Species Human
Immunogen Recombinant protein fragment of Human ROS1.
Conjugation Un-conjugated
Protein Full Name Proto-oncogene tyrosine-protein kinase ROS
Alternate Names Proto-oncogene c-Ros-1; MCF3; c-ros-1; Receptor tyrosine kinase c-ros oncogene 1; Proto-oncogene tyrosine-protein kinase ROS; c-Ros receptor tyrosine kinase; ROS; EC; Proto-oncogene c-Ros

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human ROS1+ lung carcinoma tissue


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 6098 Human ROS1

Swiss-port # P08922 Human Proto-oncogene tyrosine-protein kinase ROS

Gene Symbol ROS1
Gene Full Name ROS proto-oncogene 1 , receptor tyrosine kinase
Background This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
Function Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein
Research Area Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 264 kDa
PTM Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.

Images (1) Click the Picture to Zoom In

  • ARG66943 anti-ROS1 antibody [SQab22273] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human ROS1+ lung carcinoma tissue stained with ARG66943 anti-ROS1 antibody [SQab22273]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).