ARG45309

anti-RPS25 antibody

anti-RPS25 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Polyclonal antibody recognizes RPS25
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPS25
Antigen Species Human
Immunogen Recombinant protein containing to human RPS25.
Conjugation Un-conjugated
Alternate Names RPS25; Ribosomal Protein S25; ES25; S25; Small Ribosomal Subunit Protein ES25; 40S Ribosomal Protein S25

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 17 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 122799 Rat RPS25

GeneID: 6230 Human RPS25

GeneID: 75617 Mouse RPS25

Gene Symbol RPS25
Gene Full Name Ribosomal Protein S25
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Function The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 14 kDa
PTM Acetylation. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG45309 anti-RPS25 antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG45309 anti-RPS25 antibody at 2 μg/ml dilution.

  • ARG45309 anti-RPS25 antibody WB image

    Western blot: A549 and Hela stained with ARG45309 anti-RPS25 antibody at 0.5 μg/ml dilution.

  • ARG45309 anti-RPS25 antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45309 anti-RPS25 antibody at 1 µg/10^6 cells dilution.

  • ARG45309 anti-RPS25 antibody IHC-P image

    Immunohistochemistry: Rat liver stained with ARG45309 anti-RPS25 antibody at 2 μg/ml dilution.

  • ARG45309 anti-RPS25 antibody WB image

    Western blot: Rat liver and RH35 stained with ARG45309 anti-RPS25 antibody at 0.5 μg/ml dilution.

  • ARG45309 anti-RPS25 antibody IHC-P image

    Immunohistochemistry: Mouse liver stained with ARG45309 anti-RPS25 antibody at 2 μg/ml dilution.

  • ARG45309 anti-RPS25 antibody WB image

    Western blot: Mouse liver and NIH/3T3 stained with ARG45309 anti-RPS25 antibody at 0.5 μg/ml dilution.