ARG66766

anti-RRM1 antibody [SQab20199]

anti-RRM1 antibody [SQab20199] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab20199] recognizes RRM1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab20199
Isotype IgG
Target Name RRM1
Antigen Species Human
Immunogen Synthetic peptide within aa. 700-800 of Human RRM1.
Conjugation Un-conjugated
Alternate Names Ribonucleoside-diphosphate reductase large subunit; R1; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; RIR1; RR1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
WB: for mouse and rat samples, maybe more antibody is needed, and mouse and rat samples were tested only by WB.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Breast carcinoma tissue.
Observed Size 90 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20133 Mouse RRM1

GeneID: 6240 Human RRM1

Swiss-port # P07742 Mouse Ribonucleoside-diphosphate reductase large subunit

Swiss-port # P23921 Human Ribonucleoside-diphosphate reductase large subunit

Gene Symbol RRM1
Gene Full Name ribonucleotide reductase M1
Background This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 90 kDa

Images (2) Click the Picture to Zoom In

  • ARG66766 anti-RRM1 antibody [SQab20199] WB image

    Western blot: 1) MCF7 cell lysate, 2) HeLa cell lysate, 3) Mouse heart lysate and 4) Rat heart lysate stained with ARG66766 anti-RRM1 antibody [SQab20199] at 1:1000 dilution.

  • ARG66766 anti-RRM1 antibody [SQab20199] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human breast carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66766 anti-RRM1 antibody [SQab20199] at 18°C - 25°C for 30 minutes.

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Review for anti-RRM1 antibody [SQab20199]

Application:WB

Sample:MCF7, HeLa, Mouse heart, Rat heart

Sample Loading Amount:20 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP