ARG54882

anti-RUNX2 antibody

anti-RUNX2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes RUNX2
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RUNX2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 445-474 surrounding S533 of Human RUNX2.
Conjugation Un-conjugated
Alternate Names OSF2; Core-binding factor subunit alpha-1; CLCD; AML3; Acute myeloid leukemia 3 protein; Polyomavirus enhancer-binding protein 2 alpha A subunit; CBFA1; SL3/AKV core-binding factor alpha A subunit; PEA2aA; Osteoblast-specific transcription factor 2; SL3-3 enhancer factor 1 alpha A subunit; PEBP2-alpha A; Oncogene AML-3; CBF-alpha-1; CCD; PEBP2aA; CCD1; OSF-2; PEA2-alpha A; Runt-related transcription factor 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control CEM

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 860 Human RUNX2

Swiss-port # Q13950 Human Runt-related transcription factor 2

Gene Symbol RUNX2
Gene Full Name runt-related transcription factor 2
Background This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]
Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. [UniProt]
Cellular Localization Nucleus.
Research Area Developmental Biology antibody; Gene Regulation antibody
Calculated MW 57 kDa
PTM Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation (By similarity). Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.

Images (3) Click the Picture to Zoom In

  • ARG54882 anti-RUNX2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG54882 anti-RUNX2 antibody.

  • ARG54882 anti-RUNX2 antibody WB image

    Western blot: 35 µg of CEM cell lysate stained with ARG54882 anti-RUNX2 antibody.

  • ARG54882 anti-RUNX2 antibody FACS image

    Flow Cytometry: NCI-H460 cells stained with ARG54882 anti-RUNX2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.