anti-S100 antibody [B32.1]

anti-S100 antibody [B32.1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Bovine,Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody


Product Description

Mouse Monoclonal antibody [B32.1] recognizes S100

Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC-P
Specificity This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).
Host Mouse
Clonality Monoclonal
Clone B32.1
Isotype IgG2a
Target Name S100
Antigen Species Bovine
Immunogen bovine brain S100 protein
Conjugation Un-conjugated
Alternate Names S-100 protein beta chain; NEF; S100; Protein S100-B; S100 calcium-binding protein B; S100beta; S-100 protein subunit beta; S100-B

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Melanoma


Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 20203 Mouse S100B

GeneID: 25742 Rat S100B

GeneID: 525716 Bovine S100B

Gene Symbol S100B
Gene Full Name S100 calcium binding protein B
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
Function Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Calculated MW 11 kDa

Clone References

Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?

IHC / 

Charron G et al.
Front Aging Neurosci.,  (2014)




Insulin-like growth factor I messenger RNA and protein are expressed in the human lymph node and distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and endothelial cells.

IHC-P / Human

Oberlin D et al.
Immunology.,  (2009)




Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice.

IHC-FoFr / Mouse

Nakagomi T et al.
Eur J Neurosci.,  (2009)




Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development.

ICC/IF / Human

Bax DA et al.
PLoS One.,  (2009)




An extract (THC-002) of Ba-Wei-Die-Huang-Wan inhibits expression of tachykinins, and P2X3 and TRPV1 receptors, and inhibits ATP-induced detrusor overactivity in spontaneously hypertensive rats.

IHC-FoFr / Rat

Imamura T et al.
Neurourol Urodyn.,  (2009)