ARG64101

anti-S100A4 antibody

anti-S100A4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes S100A4
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application IHC-P, WB
Specificity Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name S100A4
Antigen Species Human
Immunogen CNEFFEGFPDKQP
Conjugation Un-conjugated
Alternate Names S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
WB0.05 - 0.2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 12 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6275 Human S100A4

Swiss-port # P26447 Human Protein S100-A4

Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Images (4) Click the Picture to Zoom In

  • ARG64101 anti-S100A4 / CAPL antibody WB image

    Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64101 anti-S100A4 / CAPL antibody at 0.03 µg/ml dilution.

  • ARG64101 anti-S100A4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64101 anti-S100A4 antibody at 2 µg/ml dilution followed by HRP-staining.

  • ARG64101 anti-S100A4 / CAPL antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64101 anti-S100A4 / CAPL antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG64101 anti-S100A4 antibody WB image

    Western blot: 35 µg of peripheral blood mononuclear cell lysates (in RIPA buffer) stained with ARG64101 anti-S100A4 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.