anti-S100A4 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
|Product Description||Goat Polyclonal antibody recognizes S100A4|
|Predict Reactivity||Ms, Rat, Dog|
|Tested Application||IHC-P, WB|
|Specificity||Both variants (NP_002952.1; NP_062427.1) represent identical protein.|
|Alternate Names||S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98|
|Application Note||WB: Recommend incubate at RT for 1h. Approx 13 kDa band observed in Human Placenta lysates (calculated MW of 11.7 kDa according to NP_002952.1and NP_062427.1). Recommended concentration: 0.03-0.1 µg/ml.
IHC-P: In paraffin embedded Human Small Intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells . Recommended concentration, 2-3 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody|
Images (2) Click the Picture to Zoom In
ARG64101 anti-S100A4 / CAPL antibody WB image
Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64101 anti-S100A4 / CAPL antibody at 0.03 µg/ml dilution.
ARG64101 anti-S100A4 / CAPL antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64101 anti-S100A4 / CAPL antibody at 2.5 µg/ml dilution followed by AP-staining.