ARG22262

anti-SAP102 antibody [S19-2]

anti-SAP102 antibody [S19-2] for Western blot,Immunohistochemistry,Immunoprecipitation,ICC/IF and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S19-2] recognizes SAP102
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
Host Mouse
Clonality Monoclonal
Clone S19-2
Isotype IgG1
Target Name SAP102
Antigen Species Rat
Immunogen Fusion protein around aa. 1-120 of Rat SAP102
Conjugation Un-conjugated
Alternate Names MRX; SAP102; NEDLG; SAP-102; Neuroendocrine-DLG; Synapse-associated protein 102; MRX90; Disks large homolog 3; PPP1R82; XLMR

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHCAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1741 Human DLG3

GeneID: 53310 Mouse DLG3

GeneID: 58948 Rat DLG3

Gene Symbol Dlg3
Gene Full Name discs, large homolog 3 (Drosophila)
Background This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
Function Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling. [UniProt]
Cellular Localization Cytoplasm, Basolateral Cell Membrane, Cell Junction, growth cone, postsynaptic cell membrane, postsynaptic density, Synapse
Calculated MW 90 kDa

Images (2) Click the Picture to Zoom In

  • ARG22262 anti-SAP102 antibody [S19-2] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22262 anti-SAP102 antibody [S19-2] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22262 anti-SAP102 antibody [S19-2] (D) Composite.

  • ARG22262 anti-SAP102 antibody [S19-2] WB image

    Western blot: Rat brain lysates stained with ARG22262 anti-SAP102 antibody [S19-2] at 1:1000 dilution.