ARG52421

anti-SAP49 antibody [3A1]

anti-SAP49 antibody [3A1] for ICC/IF,Western blot and Bovine,Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [3A1] recognizes SAP49
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 3A1
Isotype IgG2b
Target Name SAP49
Antigen Species Human
Immunogen Recombinant full length human SAP49 expressed in and purified from E. Coli.
Conjugation Un-conjugated
Alternate Names SAP 49; SF3b50; SAP49; Hsh49; AFD1; Splicing factor 3B subunit 4; SF3b49; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
WB1:1000 - 1:2000
Application Note Specific for the ~49k SAP49 protein
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10262 Human SF3B4

GeneID: 107701 Mouse SF3B4

GeneID: 295270 Rat SF3B4

Gene Symbol SF3B4
Gene Full Name splicing factor 3b, subunit 4, 49kDa
Background The spliceosome associated protein of 49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006).
Research Area Gene Regulation antibody
Calculated MW 44 kDa

Images (3) Click the Picture to Zoom In

  • ARG52421 anti-SAP49 antibody [3A1] WB image

    Western blot: HeLa lysate stained with ARG52421 anti-SAP49 antibody [3A1] showing specific immunolabeling of the ~ 49k SAP49 protein.

  • ARG52421 anti-SAP49 antibody [3A1] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG52421 anti-SAP49 antibody [3A1] showing strong granular nuclear staining of SAP49 in red and vimentin (ARG52468 anti-vimentin antibody) in green.

  • ARG52421 anti-SAP49 antibody [3A1] WB image

    Western blot: NIH/3T3 cytosol, NIH/3T3 nuclear, HeLa cytosol and HeLa nuclear fractions stained with ARG52421 anti-SAP49 antibody [3A1] (green) at 1:1000 dilution.

    The same blot was simultaneously stained with anti-GAPDH antibody (red) at 1:20000 dilution.