ARG51807

anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody

anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody for ICC/IF,Western blot and Human,Mouse,Rat

Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes SAPK / JNK phospho (Thr183 (221) / Tyr185 (223))
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Specificity The antibody detects endogenous levels of dually phosphorylated JNK1 / JNK2 at Thr183 and Tyr185, and JNK3 at Thr221 and Tyr223.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SAPK / JNK
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V) derived from Human SAPK / JNK.
Conjugation Un-conjugated
Alternate Names JNK3A; Mitogen-activated protein kinase 10; p54bSAPK; Stress-activated protein kinase 1b; JNK3; c-Jun N-terminal kinase 3; Stress-activated protein kinase JNK3; EC 2.7.11.24; SAPK1b; p493F12; MAP kinase 10; PRKM10; MAPK 10; MAP kinase p49 3F12

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25272 Rat MAPK10

GeneID: 26414 Mouse MAPK10

GeneID: 5602 Human MAPK10

Gene Symbol MAPK10
Gene Full Name mitogen-activated protein kinase 10
Background Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity
Function Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30205 NFkB Activation Antibody Panel
ARG30294 Phospho SAPK / JNK Antibody Duo (Total, pT183/Y185)
Related products:
JNK antibodies; JNK ELISA Kits; JNK Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody
Calculated MW 53 kDa
PTM Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Palmitoylation regulates subcellular location and axonal development.

Images (4) Click the Picture to Zoom In

  • ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image

    Western blot: 30 µg of HeLa cells untreated or treated with TNF-alpha at 10, 20, or 30 min. The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody at 1:500 dilution.

  • ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image

    Western blot: Extracts from C6 cells untreated or treated with anisomycin. The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.

  • ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody ICC/IF image

    Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.

  • ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image

    Western blot: Extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP). The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.

Customer's Feedback

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Review for anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody

Application:WB

Sample:HeLa cells untreated or treated with TNF-alpha

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody

Application:WB

Sample:HeLa treated with 5 ng/ml TNF-a for 1) 5 min, 2) 10 min, and 3) 20 min.

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment.

WB / Human

Shun-Cheng Wu et al.
Biomedicines.,  (2021)

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A Natural Dietary Flavone Myricetin as an α-Hemolysin Inhibitor for Controlling Staphylococcus aureus Infection.

WB / Mouse

Tingting Wang et al.
Front Cell Infect Microbiol.,  (2020)

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The fucoidan from sea cucumber Apostichopus japonicus attenuates lipopolysaccharide-challenged liver injury in C57BL/6J mice.

WB / Mouse

Yin Jiayu et al.
Journal of Functional Foods.,  (2019)

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The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

WB / Human

Du Jingchun et al.
Stem Cells International.,  (2019)

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Mice deficient in aldo-keto reductase 1a (Akr1a) are resistant to thioacetamide-induced liver injury.

WB / Mouse

Homma Takujiro et al.
Toxicol Lett.,  (2018)

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