anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody
anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
|Product Description||Rabbit Polyclonal antibody recognizes SARS-CoV-2 NSP5 / 3CLpro protein|
|Tested Application||ELISA, IHC-P, WB|
|Target Name||SARS-CoV-2 NSP5 / 3CLpro protein|
|Immunogen||A 16-amino acid synthetic peptide within the first 50 amino acids of SARS-CoV-2 NSP5 / 3CLpro protein.|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Affinity purification with immunogen.|
|Buffer||PBS and 0.02% Sodium azide.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
Images (3) Click the Picture to Zoom In
ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody at 0.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.
Strong signal of SARS-COV-2 NSP5 / 3Lpro protein was observed in macrophages of COVID-19 patient lung (left), but not in non-COVID-19 patient lung (right).
ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody WB image
Western blot: 30 ng of SARS-CoV-2 NSP5 / 3CLpro recombinant protein stained with ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody, for 1 hour at RT in 5% NFDM/TBST. Antibody dilutions: 0.5 µg/ml (left) and 1 µg/ml (right).
ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody ELISA image
Direct ELISA: SARS-CoV-2 NSP5 / 3CLpro recombinant protein as coating antigen and serial diluted ARG43303 anti-SARS-CoV-2 NSP5 / 3CLpro protein antibody as capture antibody.