anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022]

anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] for ELISA,ICC/IF,Neutralizing and Virus


Product Description Recombinant Human Monoclonal antibody [CR3022] recognizes SARS-CoV / SARS-CoV-2 Spike protein (RBD)
Tested Reactivity Virus
Tested Application ELISA, ICC/IF, Neut
Specificity This antibody binds the amino acids 318-510 in the S1 domain (RBD, Receptor Binding Domain) of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020)
Host Human
Clonality Monoclonal
Clone CR3022
Isotype IgG1
Target Name SARS-CoV / SARS-CoV-2 Spike protein (RBD)
Antigen Species Virus
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
Application Note Neutralizing: Clone CR3022 is the first anti-SARS-CoV neutralizing antibody to cross-react with SARS-CoV-2. Structural modeling has confirmed that CR3022 targets a conserved epitope between SARS-CoV and SARS-CoV2 in the RBD domain (PMID: 32245784, 32065055).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified from antibody expressing HEK293 cell culture medium.
Purity > 98% (SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


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Images (2) Click the Picture to Zoom In

  • ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] ICC/IF image

    Immunofluorescence: 293T cells were transfected with Mock or SARS-CoV-2 Spike expression plasmid. Cells were stained with ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] (green). DAPI (blue) for nuclear staining.

  • ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022] ELISA image

    ELISA: Titration of ARG66739 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody [CR3022].

    RBD = Receptor binding domain