ARG46732
anti-SERCA2 ATPase antibody [1G02]
anti-SERCA2 ATPase antibody [1G02] for Western blot and Human,Rat
Overview
| Product Description | Rabbit Monoclonal antibody [1G02] recognizes SERCA2 ATPase |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | 1G02 |
| Isotype | IgG |
| Target Name | SERCA2 ATPase |
| Immunogen | A synthetic peptide of human SERCA2 ATPase |
| Conjugation | Un-conjugated |
| Alternate Names | ATP2B; Endoplasmic reticulum class 1/2 Ca; SERCA2; SR Ca; DD; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; EC 3.6.3.8; Calcium pump 2; DAR; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; 2+ |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 115 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 0.05% BSA and 40 % Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11507 Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Swiss-port # P16615 Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 |
|---|---|
| Gene Symbol | ATP2A2 |
| Gene Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Background | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008] |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. |
Images (2) Click the Picture to Zoom In
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ARG46732 anti-SERCA2 ATPase antibody [1G02] WB image
Western blot: Hela stained with ARG46732 anti-SERCA2 ATPase antibody [1G02].
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ARG46732 anti-SERCA2 ATPase antibody [1G02] WB image
Western blot: Rat brain stained with ARG46732 anti-SERCA2 ATPase antibody [1G02].
