ARG45448
anti-SF1 antibody
anti-SF1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SF1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SF1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human SF1. |
| Conjugation | Un-conjugated |
| Alternate Names | SF1, Splicing Factor 1, ZFM1, Zinc Finger Protein 162, ZCCHC25, ZNF162, Mammalian Branch Point-Binding Protein, Zinc Finger Gene In MEN1 Locus, Transcription Factor ZFM1, MBBP, BBP, D11S636 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 68 - 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SF1 |
| Gene Full Name | Splicing Factor 1 |
| Background | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Function | Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. [UniProt] |
| Cellular Localization | Nucleus; Spliceosome. [UniProt] |
| Calculated MW | 68 kDa |
| PTM | Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. [UniProt]. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45448 anti-SF1 antibody at 2 μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: SK-OV-3 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
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ARG45448 anti-SF1 antibody FACS image
Flow Cytometry: A431 stained with ARG45448 anti-SF1 antibody at 1 µg/10^6 cells dilution.
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Rat lung stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: PC-12 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Mouse lung stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: NIH/3T3 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
