ARG46471

anti-SH3RF2 antibody

anti-SH3RF2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SH3RF2
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SH3RF2
Antigen Species Human
Immunogen A 17 amino acid synthetic peptide within the last 50 amino acids of human SH3RF2.
Conjugation Un-conjugated
Alternate Names SH3 domain containing ring finger 2; SH3RF2; HEPP1; POSHER; RNF158; PPP1R39; Putative E3 ubiquitin-protein ligase SH3RF2; Heart protein phosphatase 1-binding protein; HEPP1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -521°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 153769 Human SH3RF2

GeneID: 269016 Mouse SH3RF2

GeneID: 307472 Rat SH3RF2

Gene Symbol SH3RF2
Gene Full Name SH3 domain containing ring finger 2
Background Enables protein phosphatase 1 binding activity and ubiquitin protein ligase activity. Involved in several processes, including positive regulation of JNK cascade; protein autoubiquitination; and regulation of protein metabolic process. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Function Has E3 ubiquitin-protein ligase activity (PubMed:24130170). Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation (PubMed:22128169). Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation (PubMed:24130170). Inhibits PPP1CA phosphatase activity (PubMed:19389623, PubMed:19945436). [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 79 kDa
PTM Phosphoprotein; Ubl conjugation. [UniProt]