ARG51791

anti-SHP1 phospho (Tyr536) antibody

anti-SHP1 phospho (Tyr536) antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes SHP1 phospho (Tyr536)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SHP1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1.
Conjugation Un-conjugated
Alternate Names HCP; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase SHP-1; SH-PTP1; PTP-1C; HPTP1C; HCPH; Tyrosine-protein phosphatase non-receptor type 6; SHP-1; EC 3.1.3.48; SHP1; SHP-1L; Protein-tyrosine phosphatase 1C

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116689 Rat PTPN6

GeneID: 15170 Mouse PTPN6

GeneID: 5777 Human PTPN6

Gene Symbol PTPN6
Gene Full Name protein tyrosine phosphatase, non-receptor type 6
Background Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Function Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 68 kDa
PTM Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.

Images (2) Click the Picture to Zoom In

  • ARG51791 anti-SHP1 phospho (Tyr536) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51791 anti-SHP1 phospho (Tyr536) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51791 anti-SHP1 phospho (Tyr536) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51791 anti-SHP1 phospho (Tyr536) antibody.