anti-SHP2 antibody

anti-SHP2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human



Product Description

Rabbit Polyclonal antibody recognizes SHP2

Tested Reactivity Hu
Predict Reactivity Ms, Rat, Chk
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SHP2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 526-551 of Human SHP2.
Conjugation Un-conjugated
Alternate Names BPTP3; SHP-2; Protein-tyrosine phosphatase 2C; METCDS; PTP2C; Tyrosine-protein phosphatase non-receptor type 11; PTP-1D; CFC; PTP-2C; JMML; Shp2; Protein-tyrosine phosphatase 1D; NS1; EC; SH-PTP2; SH-PTP3; SHP2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5781 Human PTPN11

Swiss-port # Q06124 Human Tyrosine-protein phosphatase non-receptor type 11

Gene Symbol PTPN11
Gene Full Name protein tyrosine phosphatase, non-receptor type 11
Background The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. [UniProt]
Cellular Localization Cytoplasm.
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Calculated MW 68 kDa
PTM Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

Images (3) Click the Picture to Zoom In

  • ARG55762 anti-SHP2 antibody WB image

    Western blot: 30 µg of HCT116 and A549 cell lysates stained with ARG55762 anti-SHP2 antibody at 1:1000 dilution.

  • ARG55762 anti-SHP2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55762 anti-SHP2 antibody.

  • ARG55762 anti-SHP2 antibody FACS image

    Flow Cytometry: 293 cells stained with ARG55762 anti-SHP2 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.

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Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC