ARG51792

anti-SHP2 phospho (Tyr542) antibody

anti-SHP2 phospho (Tyr542) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes SHP2 phospho (Tyr542)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SHP2
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 542 (H-E-Y(p)-T-N) derived from Human SHP-2.
Conjugation Un-conjugated
Alternate Names BPTP3; SHP-2; Protein-tyrosine phosphatase 2C; METCDS; PTP2C; Tyrosine-protein phosphatase non-receptor type 11; PTP-1D; CFC; PTP-2C; JMML; Shp2; Protein-tyrosine phosphatase 1D; NS1; EC 3.1.3.48; SH-PTP2; SH-PTP3; SHP2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19247 Mouse PTPN11

GeneID: 25622 Rat PTPN11

GeneID: 5781 Human PTPN11

Gene Symbol PTPN11
Gene Full Name protein tyrosine phosphatase, non-receptor type 11
Background Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. [UniProt]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 68 kDa
PTM Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

Images (2) Click the Picture to Zoom In

  • ARG51792 anti-SHP2 phospho (Tyr542) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with starvation stained with ARG51792 anti-SHP2 phospho (Tyr542) antibody.

  • ARG51792 anti-SHP2 phospho (Tyr542) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51792 anti-SHP2 phospho (Tyr542) antibody (left) or the same antibody preincubated with blocking peptide (right).