anti-SIT antibody [SIT-01] (PE)

anti-SIT antibody [SIT-01] (PE) for Flow cytometry and Human

Signaling Transduction antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [SIT-01] recognizes SIT

Tested Reactivity Hu
Tested Application FACS
Specificity The clone SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
Host Mouse
Clonality Monoclonal
Clone SIT-01
Isotype IgG1
Target Name SIT
Antigen Species Human
Immunogen Bacterially produced recombinant intracellular fragment of human SIT.
Conjugation PE
Alternate Names gp30/40; Signaling threshold-regulating transmembrane adapter 1; SIT; SHP2-interacting transmembrane adapter protein; SIT-R; Suppression-inducing transmembrane adapter 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 27240 Human SIT1

Swiss-port # Q9Y3P8 Human Signaling threshold-regulating transmembrane adapter 1

Gene Symbol SIT1
Gene Full Name signaling threshold regulating transmembrane adaptor 1
Background SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Function Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells. [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 21 kDa
PTM Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.

Images (1) Click the Picture to Zoom In

  • ARG53961 anti-SIT antibody [SIT-01] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53961 anti-SIT antibody [SIT-01] (PE) and anti-CD3 antibody (APC).