ARG64061

anti-SNAIL antibody

anti-SNAIL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
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Overview

Product Description

Goat Polyclonal antibody recognizes SNAIL

Tested Reactivity Hu, Rat
Predict Reactivity Ms
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SNAIL
Antigen Species Human
Immunogen RKPSDPNRKPNY-C
Conjugation Un-conjugated
Alternate Names SNAH; SNAIL; SNA; dJ710H13.1; Protein sna; Protein snail homolog 1; Zinc finger protein SNAI1; SLUGH2; SNAIL1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 29 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6615 Human SNAI1

Swiss-port # O95863 Human Zinc finger protein SNAI1

Background The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
PTM Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.

Images (5) Click the Picture to Zoom In

  • ARG64061 anti-SNAIL antibody WB image

    Western blot: 20 µg of HeLa and A549 cell lysates stained with ARG64061 anti-SNAIL antibody at 1:1000 dilution.

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded breast carcinoma tissue stained with ARG64061 anti-SNAIL antibody at 1:100 dilution. Antigen Retrieval: Boil tissue section in EDTA buffer (pH 9.0).

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64061 anti-SNAIL antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64061 anti-SNAIL antibody WB image

    Western blot: 35 µg of Rat kidney lysate (in RIPA buffer) stained with ARG64061 anti-SNAIL antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded lung tissue from HGF overexpressing mouse stained with ARG64061 anti-SNAIL antibody at 1:500 dilution.

Customer's Feedback

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Review for anti-SNAIL antibody

Application:WB

Sample:3T3 cell lysate

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC