anti-SNAIL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
Goat Polyclonal antibody recognizes SNAIL
|Tested Reactivity||Hu, Ms|
|Tested Application||IHC-P, WB|
|Alternate Names||SNAH; SNAIL; SNA; dJ710H13.1; Protein sna; Protein snail homolog 1; Zinc finger protein SNAI1; SLUGH2; SNAIL1|
|Application Note||WB: Recommend incubate at RT for 1h. Approx 28 kDa band observed in lysates of cell line A431 and Human Kidney lysate (calculated MW of 29.1 kDa according to NP_005976.2). Recommended concentration: 0.1-1 µg/ml.
IHC-P: This product was successfully used in IHC on Mouse Tissue sections (lung adenocarcinoma induced by urethane) by an anonymous customer. Data obtained from a previous batch (different goat).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody|
|PTM||Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
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