ARG22294

anti-SOD1 antibody

anti-SOD1 antibody for ELISA,Immunohistochemistry,Immunoprecipitation,Western blot and Bovine,Dog,Fish,Hamster,Human,Insect,Invertebrates,Monkey,Mouse,Pig,Rabbit,Rat,Sheep,Xenopus laevis

Overview

Product Description

Rabbit Polyclonal antibody recognizes SOD1

Tested Reactivity Hu, Ms, Rat, Bov, Dog, Fsh, Hm, Insect, Invt, Mk, Pig, Rb, Sheep, Xenopus laevis
Tested Application ELISA, IHC, IP, WB
Specificity Detects ~23kDa (human) and ~19kDa (other species).
Host Rabbit
Clonality Polyclonal
Target Name SOD1
Antigen Species Human
Immunogen Human SOD1
Conjugation Un-conjugated
Alternate Names homodimer; EC 1.15.1.1; SOD; HEL-S-44; Superoxide dismutase [Cu-Zn]; ALS1; Superoxide dismutase 1; IPOA; ALS; hSod1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC1:100
IPAssay-dependent
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.0), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009313 Rabbit SOD1

GeneID: 20655 Mouse SOD1

GeneID: 24786 Rat SOD1

Gene Symbol SOD1
Gene Full Name superoxide dismutase 1, soluble
Background The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt]
Cellular Localization Cytoplasm
Calculated MW ~23 kDa (human) and ~19 kDa (other species).
PTM Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Palmitoylation helps nuclear targeting and decreases catalytic activity.
Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

Images (1) Click the Picture to Zoom In

  • ARG22294 anti-Cu/Zn-SOD antibody WB image

    Western blot: Human cell line lysates stained with ARG22294 anti-Cu/Zn-SOD antibody at 1:1000 dilution.