anti-SOX10 antibody [SQab1734]

anti-SOX10 antibody [SQab1734] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab1734] recognizes SOX10
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1734
Isotype IgG
Target Name SOX10
Antigen Species Human
Immunogen Synthetic peptide around aa. 400-500 of Human SOX10.
Conjugation Un-conjugated
Alternate Names WS4C; PCWH; DOM; Transcription factor SOX-10; WS2E; WS4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:1000 - 1:2000
ICC/IF1:2000 - 1:10000
IHC-P1:1000 - 1:2000
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 6663 Human SOX10

Swiss-port # P56693 Human Transcription factor SOX-10

Gene Symbol SOX10
Gene Full Name SRY (sex determining region Y)-box 10
Background SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]
Function SOX10 is a transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). [UniProt]
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Calculated MW 50 kDa

Images (5) Click the Picture to Zoom In

  • ARG66245 anti-SOX10 antibody [SQab1734] ICC/IF image

    Immunofluorescence: A375 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:10000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66245 anti-SOX10 antibody [SQab1734] FACS image

    Flow Cytometry: A375 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. Cells were then stained with ARG66245 anti-SOX10 antibody [SQab1734] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG66245 anti-SOX10 antibody [SQab1734] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma tissue stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:100. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66245 anti-SOX10 antibody [SQab1734] WB image

    Western blot: 10 μg of A375 cell lysate stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:1000 dilution.

  • ARG66245 anti-SOX10 antibody [SQab1734] IP image

    Immunoprecipitation: 0.4 mg of A375 cell lysate immunoprecipitated and stained with ARG66245 anti-SOX10 antibody [SQab1734]. 1) IP in A375 whole cell lysate, 2) Rabbit IgG instead of primary antibody in A375 whole cell lysate, and 3) A375 whole cell lysate, 10 μg (input).