anti-SOX10 antibody [SQab1734]
anti-SOX10 antibody [SQab1734] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1734] recognizes SOX10|
|Tested Application||FACS, ICC/IF, IHC-P, IP, WB|
|Immunogen||Synthetic peptide around aa. 400-500 of Human SOX10.|
|Alternate Names||WS4C; PCWH; DOM; Transcription factor SOX-10; WS2E; WS4|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||SRY (sex determining region Y)-box 10|
|Background||SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]|
|Function||SOX10 is a transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). [UniProt]|
SOX10 antibodies; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|Calculated MW||50 kDa|
Images (5) Click the Picture to Zoom In
ARG66245 anti-SOX10 antibody [SQab1734] ICC/IF image
Immunofluorescence: A375 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:10000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG66245 anti-SOX10 antibody [SQab1734] FACS image
Flow Cytometry: A375 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. Cells were then stained with ARG66245 anti-SOX10 antibody [SQab1734] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.
ARG66245 anti-SOX10 antibody [SQab1734] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma tissue stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:100. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
ARG66245 anti-SOX10 antibody [SQab1734] WB image
Western blot: 10 μg of A375 cell lysate stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:1000 dilution.
ARG66245 anti-SOX10 antibody [SQab1734] IP image
Immunoprecipitation: 0.4 mg of A375 cell lysate immunoprecipitated and stained with ARG66245 anti-SOX10 antibody [SQab1734]. 1) IP in A375 whole cell lysate, 2) Rabbit IgG instead of primary antibody in A375 whole cell lysate, and 3) A375 whole cell lysate, 10 μg (input).