ARG46522

anti-SPRED3 antibody

anti-SPRED3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes SPRED3
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SPRED3
Antigen Species Human
Immunogen A 16 amino acid synthetic peptide within the last 50 amino acids of human SPRED3.
Conjugation Un-conjugated
Alternate Names sprouty-related, EVH1 domain containing 3; Spred3; Eve-3; spred-3; EVE-3; Sprouty-related; EVH1 domain-containing protein 3; Spred-3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 45 kDa

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -572°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 399473 Human SPRED3

Swiss-port # Q2MJR0 Human Sprouty-related, EVH1 domain-containing protein 3

Gene Symbol SPRED3
Gene Full Name sprouty-related, EVH1 domain containing 3
Background This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminal Ena/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of a family of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly during organogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
Function Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). [UniProt]
Cellular Localization Cell membrane. [UniProt]
Calculated MW 45 kDa
PTM Phosphoprotein; Ubl conjugation; Lipoprotein; Palmitate; Methylation. [UniProt]