ARG46527
anti-SREBP2 antibody
anti-SREBP2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SREBP2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SREBP2 |
| Antigen Species | Human |
| Immunogen | A 15 amino acid synthetic peptide within the last 50 amino acids of human SREBP2. |
| Conjugation | Un-conjugated |
| Alternate Names | SREBF2; Class D basic helix-loop-helix protein 2; Sterol regulatory element-binding protein 2; bHLHd2; Sterol regulatory element-binding transcription factor 2; SREBP2; SREBP-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 128 kDa |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12772 Human Sterol regulatory element-binding protein 2 Swiss-port # Q3U1N2 Mouse Sterol regulatory element-binding protein 2 |
|---|---|
| Gene Symbol | SREBF2 |
| Gene Full Name | sterol regulatory element binding transcription factor 2 |
| Background | This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. [UniProt] |
| Calculated MW | 126 kDa |
| PTM | At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. |
Images (2) Click the Picture to Zoom In
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ARG46527 anti-SREBP2 antibody IHC-P image
Immunohistochemistry: Human spleen stained with ARG46527 anti-SREBP2 antibody.
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ARG46527 anti-SREBP2 antibody WB image
Western blot: PC-3 stained with ARG46527 anti-SREBP2 antibody.
