ARG51555

anti-STAT6 phospho (Thr645) antibody

anti-STAT6 phospho (Thr645) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes STAT6 phospho (Thr645)
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name STAT6
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of threonine 645 (P-A-T(p)-I-K) derived from Human STAT6.
Conjugation Un-conjugated
Alternate Names D12S1644; STAT6B; STAT6C; Signal transducer and activator of transcription 6; IL-4-STAT; IL-4 Stat

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6778 Human STAT6

Swiss-port # P42226 Human Signal transducer and activator of transcription 6

Gene Symbol STAT6
Gene Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Background Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Function Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. [UniProt]
Research Area Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 94 kDa
PTM Tyrosine phosphorylated following stimulation by IL4/interleukin-4 and IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.

Images (2) Click the Picture to Zoom In

  • ARG51555 anti-STAT6 phospho (Thr645) antibody WB image

    Western blot: Extracts from HeLa cells, treated with IL-4 or calf intestinal phosphatase (CIP), stained with ARG51555 anti-STAT6 phospho (Thr645) antibody.

  • ARG51555 anti-STAT6 phospho (Thr645) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51555 anti-STAT6 phospho (Thr645) antibody (left) or the same antibody preincubated with blocking peptide (right).