ARG45484
anti-STIL / SIL antibody
anti-STIL / SIL antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes STIL / SIL |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STIL / SIL |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human STIL / SIL. |
| Conjugation | Un-conjugated |
| Alternate Names | STIL; STIL Centriolar Assembly Protein; MCPH7; SIL; TAL-1-Interrupting Locus Protein; SCL-Interrupting Locus Protein; SCL/TAL1 Interrupting Locus; TAL1 (SCL) Interrupting Locus |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 170 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15468 Human SCL-interrupting locus protein Swiss-port # Q60988 Mouse SCL-interrupting locus protein homolog |
|---|---|
| Gene Symbol | STIL |
| Gene Full Name | STIL Centriolar Assembly Protein |
| Background | This gene encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V-(D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1 [UniProt] |
| Cellular Localization | Cytoplasm; Cytoskeleton. [UniProt] |
| Calculated MW | 143 kDa |
| PTM | Acetylation; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45484 anti-STIL / SIL antibody IHC-P image
Immunohistochemistry: Human gallbladder adenocarcinoma stained with ARG45484 anti-STIL / SIL antibody at 2 μg/ml dilution.
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ARG45484 anti-STIL / SIL antibody WB image
Western blot: Hela stained with ARG45484 anti-STIL / SIL antibody at 0.5 μg/ml dilution.
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ARG45484 anti-STIL / SIL antibody FACS image
Flow Cytometry: SiHa stained with ARG45484 anti-STIL / SIL antibody at 1 µg/10^6 cells dilution.
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ARG45484 anti-STIL / SIL antibody WB image
Western blot: Rat testis stained with ARG45484 anti-STIL / SIL antibody at 0.5 μg/ml dilution.
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ARG45484 anti-STIL / SIL antibody WB image
Western blot: NIH/3T3 stained with ARG45484 anti-STIL / SIL antibody at 0.5 μg/ml dilution.
