anti-STK11 / LKB1 antibody
anti-STK11 / LKB1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
|Product Description||Rabbit Polyclonal antibody recognizes STK11 / LKB1|
|Tested Reactivity||Hu, Ms|
|Tested Application||ICC/IF, IHC-P, WB|
|Target Name||STK11 / LKB1|
|Immunogen||Recombinant fusion protein corresponding to aa. 134-433 of Human STK11 / LKB1 (NP_000446.1).|
|Full Name||serine/threonine kinase 11|
|Alternate Names||hLKB1; Serine/threonine-protein kinase STK11; Liver kinase B1; Renal carcinoma antigen NY-REN-19; LKB1; EC 126.96.36.199; PJS|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Positive Control||Mouse liver|
|Calculated MW||49 kDa|
|Observed Size||57 kDa|
|Buffer||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||serine/threonine kinase 11|
|Background||This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]|
|Function||Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
Isoform 2: Has a role in spermiogenesis. [UniProt]
|Cellular Localization||Nucleus. Cytoplasm. Membrane. Mitochondrion. Note=A small fraction localizes at membranes (By similarity). Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to the mitochondrion during apoptosis. Translocates to the cytoplasm in response to metformin or peroxynitrite treatment. PTEN promotes cytoplasmic localization. Isoform 2: Nucleus. Cytoplasm. [UniProt]|
STK11 antibodies; Anti-Rabbit IgG secondary antibodies;
LKB1 deficiency in T cells promotes gut tumors
|PTM||Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity). [UniProt]|
Images (3) Click the Picture to Zoom In
ARG59080 anti-STK11 / LKB1 antibody ICC/IF image
Immunofluorescence: MCF-7 cells stained with ARG59080 anti-STK11 / LKB1 antibody.
ARG59080 anti-STK11 / LKB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG59080 anti-STK11 / LKB1 antibody at 1:100 dilution.
ARG59080 anti-STK11 / LKB1 antibody WB image
Western blot: 30 µg of Mouse liver lysate stained with ARG59080 anti-STK11 / LKB1 antibody at 1:1000 dilution.