anti-SUMO (pan) antibody

anti-SUMO (pan) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cell Biology and Cellular Response antibody


Product Description Rabbit Polyclonal antibody recognizes SUMO (pan)
Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SUMO (pan)
Antigen Species Human
Immunogen Recombinant protein encoding full length Human SUMO3.
Conjugation Un-conjugated
Alternate Names SMT3 homolog 1; SMT3A; SUMO-3; SUMO-2; Small ubiquitin-related modifier 3; SMT3H1; Ubiquitin-like protein SMT3A; Smt3B; Smt3A

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293


Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 499417 Rat SUMO3

GeneID: 6612 Human SUMO3

Swiss-port # P55854 Human Small ubiquitin-related modifier 3

Swiss-port # Q5XIF4 Rat Small ubiquitin-related modifier 3

Gene Symbol SUMO3
Gene Full Name small ubiquitin-like modifier 3
Background This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]
Function Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). [UniProt]
Cellular Localization Cytoplasm. Nucleus. Nucleus, PML body
Research Area Cell Biology and Cellular Response antibody
Calculated MW 12 kDa
PTM Polymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.

Images (3) Click the Picture to Zoom In

  • ARG54869 anti-SUMO (pan) antibody ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54869 anti-SUMO (pan) antibody (green) at 1:25 dilution, 1 h at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). DAPI (blue) for nuclear staining.

  • ARG54869 anti-SUMO (pan) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human bladder tissue stained with ARG54869 anti-SUMO (pan) antibody at 1:100 dilution.

  • ARG54869 anti-SUMO (pan) antibody WB image

    Western blot: 35 µg of 293 cell lysate stained with ARG54869 anti-SUMO (pan) antibody.