anti-SUMO2 + SUMO3 antibody
anti-SUMO2 + SUMO3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
|Product Description||Rabbit Polyclonal antibody recognizes SUMO2 + SUMO3|
|Tested Reactivity||Hu, Ms|
|Predict Reactivity||Bov, Rat, Chk, Hm, Mk, Pig, Xenopus, Zfsh|
|Tested Application||ICC/IF, IHC-P, WB|
|Target Name||SUMO2 + SUMO3|
|Immunogen||KLH-conjugated synthetic peptide corresponding to aa. 49-81 (C-terminus) of Human SUMO2 / SUMO3.|
|Alternate Names||SMT3 homolog 1; SMT3A; SUMO-3; SUMO-2; Small ubiquitin-related modifier 3; SMT3H1; Ubiquitin-like protein SMT3A; Smt3B; Smt3A|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Purification with Protein G.|
|Buffer||PBS and 0.09% (W/V) Sodium azide|
|Preservative||0.09% (W/V) Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
Swiss-port # P55854 Human Small ubiquitin-related modifier 3
Swiss-port # Q9Z172 Mouse Small ubiquitin-related modifier 3
|Gene Full Name||small ubiquitin-like modifier 3|
|Background||This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]|
|Function||Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). [UniProt]|
|Cellular Localization||Cytoplasm. Nucleus. Nucleus, PML body|
|Research Area||Cell Biology and Cellular Response antibody|
|Calculated MW||12 kDa|
|PTM||Polymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
Images (3) Click the Picture to Zoom In
ARG54893 anti-SUMO2 + SUMO3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54893 anti-SUMO2 + SUMO3 antibody (green) at 1:100 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
ARG54893 anti-SUMO2 + SUMO3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG54893 anti-SUMO2 + SUMO3 antibody.
ARG54893 anti-SUMO2 + SUMO3 antibody WB image
Western blot: 35 µg of 293T cell lysate stained with ARG54893 anti-SUMO2 + SUMO3 antibody at 1:1000 dilution.