ARG45780
anti-Sec31A antibody
anti-Sec31A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Sec31A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Sec31A |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Sec31A. |
| Conjugation | Un-conjugated |
| Alternate Names | SEC31A; SEC31 Homolog A, COPII Coat Complex Component; ABP125; ABP130; KIAA0905; SEC31L1; Protein Transport Protein Sec31A; SEC31-Related Protein A; SEC31-Like Protein 1; Web1-Like Protein; SEC31 Homolog A, COPII Coating Complex Component; Sec31 Homolog A (S. Cerevisiae); SEC31-Like 1 (S. Cerevisiae); Yeast Sec31p Homolog; HSPC275; HSPC334; NEDSOSB; HPBKS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 133 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SEC31A |
| Gene Full Name | SEC31 Homolog A, COPII Coat Complex Component |
| Background | The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| Function | Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). [UniProt] |
| Cellular Localization | Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Membrane. [UniProt] |
| Calculated MW | 133 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG45780 anti-Sec31A antibody IHC-P image
Immunohistochemistry: Human renal cancer stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.
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ARG45780 anti-Sec31A antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45780 anti-Sec31A antibody at 5 μg/ml dilution.
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ARG45780 anti-Sec31A antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.
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ARG45780 anti-Sec31A antibody WB image
Western blot: HepG2 and U-87MG stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.
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ARG45780 anti-Sec31A antibody FACS image
Flow Cytometry: HL-60 stained with ARG45780 anti-Sec31A antibody at 1 µg/10^6 cells dilution.
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ARG45780 anti-Sec31A antibody IHC-P image
Immunohistochemistry: Rat colon stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.
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ARG45780 anti-Sec31A antibody WB image
Western blot: PC-12 stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.
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ARG45780 anti-Sec31A antibody WB image
Western blot: NIH/3T3 stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.
