anti-Sialyl Tn antibody [STn 219]

anti-Sialyl Tn antibody [STn 219] for Flow cytometry,ICC/IF,Immunohistochemistry and Human

Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody
publication_link Publication1


Product Description Mouse Monoclonal antibody [STn 219] recognizes Sialyl Tn
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC
Specificity This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
Host Mouse
Clonality Monoclonal
Clone STn 219
Isotype IgG1, kappa
Target Name Sialyl Tn
Immunogen Ovine submaxillary mucin (OSM)
Epitope NeuAc α GalNacOSer/Thr
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
ICC/IF1:10 - 1:20
IHC1:50 - 1:100
Application Note IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears.
IHC: Use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature (1:50 - 1:100).
IF, FITC~conjugated antibodies 10-20 µg/ml (1:10 - 1:20), incubatein the dark for 2 hours at RT or overnight at 4°C.
Flow: FITC~conjugated antibodies, 0.2-1.0 µg/0.1 ml (1:200 - 1:1000).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human gastrointestinal tumor, prostate, ovary ca


Form Liquid
Buffer PBS (pH 7.4), 1% BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Background Sialsyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialsyl Tn antigen has a limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas.
Cellular Localization Cytoplasmic, apical membrane, luminal
Highlight Related Antibody Duos and Panels:
ARG30015 Tn Antigen Antibody Duo (sTn, Tn)
Related products:
Sialyl Tn antibodies; Sialyl Tn Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody
Calculated MW several HM weight glycoproteins

Images (1) Click the Picture to Zoom In

  • ARG53971 anti-Sialyl Tn antibody [STn 219] FACS image

    Flow Cytometry: MKN45 cells (orange, positive cell line) and KATO III cells (black, negative cell line) stained with ARG53971 anti-Sialyl Tn antibody [STn 219].

Specific References

Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.

ICC/IF / Human

Elizabeth Ngoc Hoa Tran et al.
ACS Infect Dis.,  (2020)