ARG55242

anti-Slug antibody

anti-Slug antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
publication_link Publication1

Overview

Product Description

Rabbit Polyclonal antibody recognizes Slug

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Slug
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 98-129 (Center) of Human Slug.
Conjugation Un-conjugated
Full Name snail family zinc finger 2
Alternate Names Protein snail homolog 2; SNAIL2; WS2D; Neural crest transcription factor Slug; Zinc finger protein SNAI2; SLUG; SLUGH1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20583 Mouse SNAI2

GeneID: 25554 Rat SNAI2

GeneID: 6591 Human SNAI2

Gene Symbol SNAI2
Gene Full Name snail family zinc finger 2
Background SLUG is a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects.
Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
PTM GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.

Images (3) Click the Picture to Zoom In

  • ARG55242 anti-Slug antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG55242 anti-Slug antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI (blue) for nuclear staining.

  • ARG55242 anti-Slug antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Breast tissue stained with ARG55242 anti-Slug antibody.

  • ARG55242 anti-Slug antibody WB image

    Western blot: 20 µg of Mouse heart lysate stained with ARG55242 anti-Slug antibody at 1:2000 dilution.

Specific References

Rap1A promotes esophageal squamous cell carcinoma metastasis through the AKT signaling pathway.

WB / Human

Li Qinfang et al.
Oncol Rep.,  (2019)

publication_link

 

hr_line