ARG54653

anti-Smac / Diablo antibody

anti-Smac / Diablo antibody for ELISA,Immunohistochemistry,Immunoprecipitation,Western blot,ICC/IF and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Smac / Diablo
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Smac / Diablo
Immunogen Synthetic peptide (16 aa) within the last 50 aa of Mouse Smac.
Conjugation Un-conjugated
Alternate Names Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IF10 μg/mL
IHC2 μg/mL
IPAssay-Dependent
WB1 μg/mL
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse Heart Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56616 Human DIABLO

GeneID: 66593 Mouse DIABLO

Swiss-port # Q9JIQ3 Mouse Diablo homolog, mitochondrial

Swiss-port # Q9NR28 Human Diablo homolog, mitochondrial

Gene Symbol Diablo
Gene Full Name diablo homolog (Drosophila)
Background Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues. |
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 27 kDa
PTM Ubiquitinated by BIRC7/livin.

Images (3) Click the Picture to Zoom In

  • ARG54653 anti-Smac antibody ICC/IF image

    Immunofluorescence: mouse spleen cells stained with ARG54653 anti-Smac antibody at 10 μg/ml.

  • ARG54653 anti-Smac antibody IHC image

    Immunohistochemistry: mouse spleen tissue stained with ARG54653 anti-Smac antibody at 2 μg/ml.

  • ARG54653 anti-Smac antibody WB image

    Western blot: mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate (C) stained with ARG54653 anti-Smac antibody at 1 μg /ml.