anti-Smad 7 antibody
anti-Smad 7 antibody for Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes Smad 7|
|Predict Reactivity||Ms, Rat|
|Specificity||This antibody detects SMAD6 at 53 kDa on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.|
|Target Name||Smad 7|
|Immunogen||Synthetic peptides around aa. 12-29 (RLWRSRAPGGEDEEEGAG) and aa. 36-50 (ELRGEGATDSRAHGA) of Human SMAD7. This sequence is 100% or highly conserved between Human and multiple species.|
|Alternate Names||Mothers against decapentaplegic homolog 8; MADH8; MADH7; Smad7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 7; hSMAD7; Mothers against DPP homolog 8; MAD homolog 8; CRCS3; SMAD 7; MAD homolog 7; SMAD family member 7|
|Application Note||WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein G.|
|Buffer||100 μl PBS, 0.05% BSA and 0.05% Sodium azide|
|Preservative||0.05% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||SMAD family member 7|
|Background||The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]|
|Function||Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). [UniProt]|
|Research Area||Gene Regulation antibody; Signaling Transduction antibody|
|Calculated MW||46 kDa|
|PTM||Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation (PubMed:14657019, PubMed:16601693). In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation (PubMed:11278251).
Acetylation prevents ubiquitination and degradation mediated by SMURF1.
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