ARG45527
anti-Sprouty 4 / Spry-4 antibody
anti-Sprouty 4 / Spry-4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Sprouty 4 / Spry-4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Sprouty 4 / Spry-4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Sprouty 4 / Spry-4. |
| Conjugation | Un-conjugated |
| Alternate Names | SPRY4; Sprouty RTK Signaling Antagonist 4; Protein Sprouty Homolog 4; Sprouty Homolog 4 (Drosophila); Sprouty Homolog 4; Spry-4; HH17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPRY4 |
| Gene Full Name | Sprouty RTK Signaling Antagonist 4 |
| Background | This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014] |
| Function | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. [UniProt] |
| Cellular Localization | Cell membrane; Cell projection; Cytoplasm. [UniProt] |
| Calculated MW | 33 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45527 anti-Sprouty 4 / Spry-4 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45527 anti-Sprouty 4 / Spry-4 antibody at 2 μg/ml dilution.
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ARG45527 anti-Sprouty 4 / Spry-4 antibody WB image
Western blot: PC-3 stained with ARG45527 anti-Sprouty 4 / Spry-4 antibody at 0.5 μg/ml dilution.
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ARG45527 anti-Sprouty 4 / Spry-4 antibody FACS image
Flow Cytometry: PC-3 stained with ARG45527 anti-Sprouty 4 / Spry-4 antibody at 1 µg/10^6 cells dilution.
