ARG51724

anti-Stathmin 1 phospho (Ser16) antibody

anti-Stathmin 1 phospho (Ser16) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Stathmin 1 phospho (Ser16)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Stathmin 1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 16 (R-A-S(p)-G-Q) derived from Human Stathmin 1.
Conjugation Un-conjugated
Alternate Names PP17; Prosolin; Stathmin; Protein Pr22; PR22; Lag; C1orf215; PP19; pp19; SMN; OP18; Leukemia-associated phosphoprotein p18; LAP18; pp17; Oncoprotein 18; Phosphoprotein p19; Op18; Metablastin

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16765 Mouse STMN1

GeneID: 29332 Rat STMN1

GeneID: 3925 Human STMN1

Gene Symbol STMN1
Gene Full Name stathmin 1
Background Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear
Function Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity). [UniProt]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 17 kDa
PTM Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.

Images (3) Click the Picture to Zoom In

  • ARG51724 anti-Stathmin 1 phospho (Ser16) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with Nocodazol stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody.

  • ARG51724 anti-Stathmin 1 phospho (Ser16) antibody WB image

    Western blot: Extracts from HeLa cells, treated with Noc or calf intestinal phosphatase (CIP), stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody.

  • ARG51724 anti-Stathmin 1 phospho (Ser16) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody (left) or the same antibody preincubated with blocking peptide (right).