ARG45753
anti-Sufu antibody
anti-Sufu antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Sufu |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Sufu |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Sufu. |
| Conjugation | Un-conjugated |
| Alternate Names | suppressor of fused homolog (Drosophila); Suppressor of fused homolog; SUFUXL; PRO1280; SUFUH |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 54 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SUFU |
| Gene Full Name | suppressor of fused homolog (Drosophila) |
| Background | SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700) |
| Function | Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 54 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45753 anti-Sufu antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45753 anti-Sufu antibody at 5 μg/ml dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: HEL stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
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ARG45753 anti-Sufu antibody FACS image
Flow Cytometry: U937 stained with ARG45753 anti-Sufu antibody at 1 µg/10^6 cells dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: Rat brain stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: Mouse brain stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
