ARG54373

anti-Survivin antibody

anti-Survivin antibody for Immunohistochemistry,Western blot and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes Survivin

Tested Reactivity Ms
Tested Application IHC, WB
Specificity This antibody recognizes mouse Survivin (17kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Survivin
Antigen Species Mouse
Immunogen Synthetic peptide corresponding to aa 128-140 of mouse Survivin (accession no. BAA28266).
Conjugation Un-conjugated
Alternate Names API4; Apoptosis inhibitor 4; EPR-1; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse spleen

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11799 Mouse BIRC5

Swiss-port # O70201 Mouse Baculoviral IAP repeat-containing protein 5

Gene Symbol Birc5
Gene Full Name baculoviral IAP repeat-containing 5
Background Apoptosis can be prevented by the inhibitor of apoptosis proteins (IAPs). IAPs constitute a conserved gene family that binds to and inhibits cell death proteases. A novel IAP has been identified and designated Survivin. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is expressed predominantly in tissues of embryos, transformed cell lines, and many human cancers.
Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7 (By similarity). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody
Calculated MW 16 kDa
PTM Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Images (2) Click the Picture to Zoom In

  • ARG54373 anti-Survivin antibody WB image

    Western Blot: mouse spleen stained with ARG54373 anti-Survivin antibody at 1 μg/ml dilution.

  • ARG54373 anti-Survivin antibody IHC image

    Immunohistochemistry: mouse spleen stained with ARG54373 anti-Survivin antibody at 10 μg/ml dilution.