ARG52429

anti-Synapsin 1 phospho (Ser9) antibody

anti-Synapsin 1 phospho (Ser9) antibody for Immunohistochemistry,Western blot and Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Synapsin 1 phospho (Ser9)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Xenopus laevis, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Synapsin 1
Antigen Species Rat
Immunogen KLH-conjugated phosphospecific peptide around Ser9 of Rat Synapsin 1.
Conjugation Un-conjugated
Alternate Names SYNI; Brain protein 4.1; Synapsin-1; SYN1a; SYN1b; Synapsin I

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:500
WB1:1000
Application Note Specific for ~78k synapsin I doublet protein phosphorylated at Ser9 . The antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with λ phosphatase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24949 Rat SYN1

Swiss-port # P09951 Rat Synapsin-1

Gene Symbol Syn1
Gene Full Name synapsin I
Background Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).
Function Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. [UniProt]
Research Area Neuroscience antibody
Calculated MW 74 kDa
PTM Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Images (2) Click the Picture to Zoom In

  • ARG52429 anti-Synapsin 1 phospho (Ser9) antibody ICC/IF image

    Immunofluorescence: Cultured Mouse caudate neurons stained with ARG52429 anti-Synapsin 1 phospho (Ser9) antibody showing Synapsin 1 when phosphorylated at Ser9.

  • ARG52429 anti-Synapsin 1 phospho (Ser9) antibody WB image

    Western blot: Rat cortex lysate showing specific immunolabeling of ~78 kDa Synapsin 1 phosphorylated at Ser9 (Control) stained with ARG52429 anti-Synapsin 1 phospho (Ser9) antibody. Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase).