ARG52433

anti-Synaptobrevin antibody [SP10]

anti-Synaptobrevin antibody [SP10] for Western blot,Immunohistochemistry and Bovine,Human,Mouse,Pig,Rat,Rabbit,Hamster

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [SP10] recognizes Synaptobrevin
Tested Reactivity Hu, Ms, Rat, Bov, Hm, Pig, Rb
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone SP10
Isotype IgM
Target Name Synaptobrevin
Antigen Species Human
Immunogen Synaptic immunoprecipitate (crude) from human brain
Conjugation Un-conjugated
Alternate Names Vesicle-associated membrane protein 1; Synaptobrevin-1; SYB1; VAMP-1; SPAX1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:100
WB1:1000
Application Note The antibody is specific for the ~16kDa synaptobrevin protein in Western blots of Rat brain lysate. The antibody has also been demonstRated to work in immunohistochemistry on formalin fixed, vibRatome sections, but does not work on paraffin sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification IgM Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22317 Mouse VAMP1

GeneID: 25624 Rat VAMP1

GeneID: 513621 Bovine VAMP1

Gene Symbol VAMP1/2
Gene Full Name vesicle-associated membrane protein 1 (synaptobrevin 1)
Background Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer’s disease (Sze et al., 2000).
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 13 kDa

Images (1) Click the Picture to Zoom In

  • ARG52433 anti-Synaptobrevin antibody [SP10] WB image

    Western blot: Rat brain lysate showing specific immunolabeling of the ~ 16k synaptobrevin protein stained with ARG52433 anti-Synaptobrevin antibody [SP10].