ARG56230
anti-Synaptophysin antibody
anti-Synaptophysin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Synaptophysin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Synaptophysin |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 225-253 (C-terminus) of Human Synaptophysin. |
| Conjugation | Un-conjugated |
| Alternate Names | Synaptophysin; MRXSYP; Major synaptic vesicle protein p38; MRX96 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Mouse cerebellum |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SYP |
| Gene Full Name | synaptophysin |
| Background | This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011] |
| Function | Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome |
| Calculated MW | 34 kDa |
| PTM | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. |
Images (4) Click the Picture to Zoom In
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ARG56230 anti-Synaptophysin antibody ICC/IF image
Immunofluorescence: Human brain cells stained with ARG56230 anti-Synaptophysin antibody (green). DAPI (blue) for nuclear staining.
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ARG56230 anti-Synaptophysin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG56230 anti-Synaptophysin antibody.
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ARG56230 anti-Synaptophysin antibody WB image
Western blot: 35 µg of Mouse cerebellum lysate stained with ARG56230 anti-Synaptophysin antibody.
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ARG56230 anti-Synaptophysin antibody FACS image
Flow Cytometry: Neuro-2a cells stained with ARG56230 anti-Synaptophysin antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
