anti-Synaptophysin antibody [SQab19147]

anti-Synaptophysin antibody [SQab19147] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat



Product Description Recombinant Rabbit Monoclonal antibody [SQab19147] recognizes Synaptophysin
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab19147
Isotype IgG
Target Name Synaptophysin
Antigen Species Human
Immunogen Synthetic peptide within aa. 213-313 of Human Synaptophysin.
Conjugation Un-conjugated
Alternate Names Synaptophysin; MRXSYP; Major synaptic vesicle protein p38; MRX96

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 20977 Mouse SYP

GeneID: 24804 Rat SYP

GeneID: 6855 Human SYP

Gene Symbol SYP
Gene Full Name synaptophysin
Background This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). [UniProt]
Cellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. [UniProt]
Calculated MW 34 kDa
PTM Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66629 anti-Synaptophysin antibody [SQab19147] WB image

    Western blot: 20 μg of Mouse brain and Rat brain lysates stained with ARG66629 anti-Synaptophysin antibody [SQab19147] at 1:500 dilution.

  • ARG66629 anti-Synaptophysin antibody [SQab19147] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human glioma tissue stained with ARG66629 anti-Synaptophysin antibody [SQab19147]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).

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Sample:Mouse brain and Rat brain

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC